This course is designed to train technicians for the hand-on techniques in disease diagnosis and treatment of major aquatic species. The aims of this course are to provide an understanding of current agents that cause diseases in shrimp in aquaculture, promotes correct diagnoses and recommend some appropriate control measure
SSNS Training Course VET1
Aquatic animal (shrimp and fish) diseases diagnosis
Background
In the Mekong Delta of Vietnam, freshwater fish (mainly catfish) and marine shrimp (mainly black tiger shrimp and white-leg shrimp) are the major commercial species. Catfish and shrimp culture are of the leading aquaculture industries which significantly contribute to the country economy. However, their explosive development with intensified density of culture practices have been troubled by many obstacles and challenges including problems on diseases and seed quality, environmental sustainability, export-import trading barriers, and other global and regional socioeconomic problems of which disease issue was one of the most concerned problems. Recently, many aquaculture companies need more well-trained technicians for their fish and shrimp disease control. This course is designed to train technicians for the hand-on techniques in disease diagnosis and treatment of major aquatic species. The aims of this course are to provide an understanding of current agents that cause diseases in shrimp in aquaculture, promotes correct diagnoses and recommend some appropriate control measure.
Learning outcome
Upon completion of this course, learners should be able to:
1. collect samples (in the field) and test for aquaculture environmental parameters
2. count the bacteria (total bacteria, Vibrio) in aquaculture environment and in the intestine of shrimp, catfish
3. use microscopy to check for normal and abnormal hepatopancreas and intestine cells of shrimp
Lectures – Five days (2 hours every day) – Classroom and field
Day 1: Lecture on shrimp sample collection (in field):
Day 2: Lecture on Shrimp bacteriological assessment
Day 3: Lecture on sample collection (in field):
Day 4: Lecture on catfish bacteriological assessment
Day 5: Lecture on preservation techniques
Practical – Five days (6 hours every day) – Shrimp and catfish farms
Day 1: In shrimp farm
• Shrimp sample (healthy and diseased) collection:
• Hepatopancreas analysis (in field)
• Intestine content analysis (in field)
• Vibrio count in shrimp sample
• Vibrio count in pond (water + mud)
• Shrimp health assessment (in field)
• environmental parameter testing (pond water)
Assessment
Day 2: In laboratory
• Microbiological laboratory preparation
• Hepatopancreas assessment (microscopy)
• Intestine content assessment (microscopy)
• Vibrio count in shrimp sample (plate count)
• Vibrio count in pond (water + mud) (plate count)
• Recommend some appropriate control measures
Assessment
Day 3: In catfish farm
• Catfish sample (healthy and diseased) collection:
• Parasite analysis of catfish
• Intestine content analysis (in field)
• Total bacteria count in catfish sample
• Total bacteria count in pond (water + mud)
• Catfish health assessment (in field)
• Environmental parameter testing (pond water)
Assessment
Day 4: In laboratory
• Microbiological laboratory preparation
• Intestine content assessment (microscopy)
• Total bacteria count in catfish sample (plate count)
• Total bacteria count in pond (water + mud) (plate count)
• Recommend some appropriate control measures
Assessment
Day 5: In laboratory
• Cold and freezing preservation: Shrimp sample, catfish samples, pond water sample, mud sample, isolated bacteria preservation in laboratory for long and cold or freezing storage.
Assessment
Final report: each trainee is required to prepare a report on what they practice at the farm and laboratory – The length of the report may vary accordingly but should not be over 10 pages.
Presentation of results by the group of trainees (Seminar Room)
- At the end of practical session, group presentation will be organized, in which they will discuss what lesson they may technically learn from the training.
- Wrap-up by Instructor (1 hour)
Feedback and evaluation by participants
Resource persons: Nguyen Huu Thinh, Truyen Nha Dinh Hue, Nguyen Hoang Nam Kha
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